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We excluded 43,945 individuals under the age of 40, 21 pregnant participants, 3,008 with missing niacin intake data, and 3,303 with missing covariate data

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Electrophoresis was performed at 4 C and 100 V using anode and cathode buffers, followed by protein transfer onto PVDF membranes and methanol decolorization

At the same time, metabolic shift towards aerobic glycolysis, altered lipid metabolism, and glutamine dependency due to oncogenic signaling and hypoxic stress, further promotes tumor survival and therapy resistance (123)