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Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h
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[Google Scholar] Valli J
A note on specific brands: Some pre-filled HGH pens ship with a cartridge-specific diluent that is neither standard bacteriostatic water nor sterile water
