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(level 4) Heating further, to hot liquid water, now leads to breaking both hydrogen bonds and van der Waals interactions, leading to reduced density, as would be observed in a normal simpler liquid

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Safranin O Staining Pellets were fixed overnight at 4C in 10% buffered formalin (Fisher Chemical, Hampton, NH, United States), dehydrated in gradient ethanol, cleared in xylene, and then embedded in paraffin
