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Focusing on direct transcriptional targets of NHR-49 with its established roles in lipid metabolism 34,35,36,37,38,39,40,41 , we confirmed that fasting-induced activation of the mitochondrial medium-chain acyl-CoA ligase ( acs-2) and repression of the stearoyl-CoA desaturase ( fat-7) were both abrogated in the loss-of-function nhr-49(nr2041) mutant animals (Fig

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