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Description
Modulation of the brain-gut axis and the NO pathway

Biotin-MYC was bound to streptavidin-coated 96-well plates (Pierce), and MYC levels were measured by enzyme-linked immunosorbent assay (ELISA) using rabbit anti-MYC antibody and peroxidase-conjugated anti-rabbit antibody followed by a peroxidase colour reaction with TMB ELISA substrate solution (eBioscience)
Activin receptor-like kinase 7 suppresses lipolysis to accumulate fat in obesity through downregulation of peroxisome proliferator-activated receptor and C/EBP

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Nearly all of the evidence behind it comes from animal and in-vitro (preclinical) studies , not from completed human efficacy trials
