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Saliva tests, often used in roadside or employment screenings, reflect recent alcohol use but may vary by test type and individual hydration levels

Immunocytochemistry For immunocytochemistry, cultured cortical neurons were fixed between DIV21 and DIV25 in 4% paraformaldehyde (PFA)/2% sucrose supplemented with 0.0025% glutaraldehyde (cat#: 16000, Electron Microscopy Sciences, Hatfield, PA) in PBS for 8 min at room temperature

The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity

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doi: 10.1038/mi.2008.41
