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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

10.1136/gut.21.9.793 Gut 179 RoshanravanN.MahdaviR.AlizadehE.JafarabadiM

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doi: 10.3389/fmed.2025.1565275 Received 22 January 2025 Accepted 20 May 2025 Published 13 June 2025 Corrected 14 July 2025 Volume 12 - 2025 Edited by Jlio Belo Fernandes, Egas Moniz Center for Interdisciplinary Research (CiiEM), Portugal Reviewed by Ajith Sominanda, Qatar University, Qatar Ricardo Antunes, Egas Moniz Center for Interdisciplinary Research (CiiEM), Portugal Updates Copyright 2025 Anghelescu, Munteanu, Spinu, Ciobanu, Popescu, Cioca, Andone, Stoica, Mandu, Rebedea, Giuvara, Malaelea, Vladulescu-Trandafir, Morcov and Onose