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Induction of apoptosis was detected by Propidium Iodide (PI, 1 g/mL) (Sigma Aldrich) and Annexin-V-APC (Immunotools) staining, followed by flow cytometry analysis, using a BD FACSCantoII flow cytometer

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The disorder is marked by a spectrum of symptoms, including hallucinations (commonly auditory), delusions (fixed false beliefs), disorganized thinking, and negative symptoms such as social withdrawal and lack of motivation

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Sperm are produced in the testes within seminiferous tubules, regulated by two key hormones: luteinising hormone (LH), which stimulates testosterone production, and follicle-stimulating hormone (FSH), which directly drives sperm maturation