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Description
The 2 Ct method was employed to determine the fold change in the RNA expression of one sample compared to the calibration sample

In situ analysis of methylglyoxal metabolism in Saccharomyces cerevisiae
F.TinelliE.TrtzmllerM.et al (2018)
But those changes are typically gastric and appetite-related, driven by the GLP-1 component

Flow cytometry was performed on a BD FACSCalibur flow cytometer, and data analysis was performed with the accompanying software
