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It plays a crucial role in energy metabolism by transporting long-chain fatty acids into the mitochondria, where they are converted into energy

While research into this topic is currently limited, current studies indicate that the microbiota has a decisive role in the synthesis of TMAO from dietary choline and carnitine

The cultures were inoculated to an OD 600 of 0.1 from precultures that were made in standard M9 medium supplemented with 1% (v/v) LB medium and placed in a shaking incubator (37C, 180 min 1 shaking frequency, 25 mm eccentricity)

PMID 25827527