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Various factors, including hyperglycemia, lipid accumulation, oxidative stress, hypoxia, RAAS, ER stress, inflammation, EMT and programmed cell death, have been shown to induce renal tubular injury and contribute to the progression of DKD

The cells were observed under a thunder DMI8 confocal microscope (Leica), and the fusion activity was assessed by calculating the relative Zsgreen fusion area through ImageJ software

Eosinophils are associated with the reinstatement of tissue homeostasis in some tissues, playing critical roles in driving regenerative responses in models of liver 90 and muscle injury 91 in an IL-4 dependent manner