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According to parabola proportion between inhibition percentage and enzyme activity, different samples have different sample volumes and different sample concentration, you need to determine an optimal sample volume and an optimal sample concentration before measure GPX activity in a new sample

Purified GST-tagged recombinant proteins were diluted to a final concentration of 175 nM in 4 mL of binding buffer (PBS-T containing 0.45% BSA, 0.5 mM EDTA, 0.1 mM DTT, and 10% glycerol), and applied to the array surface

doi:10.1074/jbc.M305568200

Error bars indicate the confidence intervals from each flux analysis

Injection Site Rotation Rotate injection sites to prevent lipodystrophy (fat tissue changes at the injection site)