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Yang, ShanghaiTech University, China Updates Copyright 2026 Zhao, Zhu, Tang, Zhu and Liu

To complement egtA 26933 , a PCR fragment containing the egtA locus including promoter and terminator was amplified using primers oAfDUF323.5 and oAfDUF323.6 and then inserted into the pCR 2.1-TOPO TA vector

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10.1016/j.cell.2011.10.026 Cell

Animal preparation for BIRDS temperature analysis The animals were anaesthetized with 3% isoflurane in an induction chamber and then kept at 23% during surgery