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In general, three components of the ghrelin system have been targeted in the search for an elusive pharmacological compound that will contribute to weight loss

doi: 10.1016/j.cmet.2011.11.004

In some cases, the sulfur of the cysteine S -conjugate may be oxidized by flavin-dependent monooxygenases (FMOs), but in other cases, the oxidation of the sulfur may occur after release of a thiol by a -lyase reaction and thiomethylation (see the next section)

For measuring intracellular GSH content of different kinds of cells, cells were collected after 24 h with rinsed twice by PBS and the cell numbers were calculated using a cell counter

as NADPH oxidase and ion channel inhibitors