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The combined treatment demonstrated significantly greater efficacy than standard-dose pregabalin or acetyl-l-carnitine alone, in terms of both pain symptoms measured by improvement on the VAS score (+12.8%) and neurosensory symptoms measured by the DN4 tool (+22%)

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Additionally, while ultracentrifugation is a common method of EV isolation from biological fluids 61 , this method carries a substantial risk of sample contamination with lipoproteins and other biomolecules that are co-isolated and are fused with EVs under these conditions

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