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Safety, Side Effects and Contraindications Thymosin Alpha-1 is described as generally well tolerated, with mild and temporary effects that may include localised redness or mild discomfort at the injection site, temporary fatigue, headache or low grade fever, and less commonly mild flu like symptoms or transient changes in liver enzymes

doi: 10.1124/jpet.108.150276

In another study, Armstrong et al

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The sample was centrifuged at 10,000 g for 10 min, and 200 L of the supernatant was filtered through a protein precipitation plate
