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Description
The selected particles were then subjected to reference-based motion correction in cryoSPARC (v4.5.0), followed by several rounds of heterogeneous refinement using volumes of different states of SLC33A1, resulting in a final stack of 215,071 particles
Tibblin, G., Eriksson, M., Cnattingius, S

Turn the needle and syringe upside down and carefully press the plunger until a small droplet of B12 appears at the end of the needle

Slapped those bad boys on in May 2018 and next came the windows

Another hypothesis is one resulting in ischemia due to paracetamol, which can be due to the depletion of sulfydryl groups
