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The peptide looked fine, tested pure via mass spec for amino acid content, but showed no biological activity in cell culture assays

Dosing & Reconstitution Guide A single practical dilution with simple unit math, step by step Standard / Gradual Approach (3 mL = ~3.33 mg/mL) Reconstitute: Add 3.0 mL bacteriostatic water to one 10 mg vial final concentration ~3.33 mg/mL

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What they found: Dose-dependent stimulation of type II collagen (up to 2.5 baseline at 100 nM) Stimulation of aggrecan (up to 1.8 baseline) Inhibition of MMP-13 by ~40 % Partial protection against IL-1-induced matrix degradation Why it matters: It opened a completely new research area for AOD-9604 beyond lipolysis