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Description
After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice
Cochrane Gynaecology and Fertility Group, editor

Moreover, individuals with LC had higher levels of antibodies to SARS-CoV-2, EBV and VZV antigens

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Alterations in the levels of colon proteins, such as trefoil factor 3 (Tff3), mucin 1 (Muc1), and Muc2, increase susceptibility to chronic inflammation, indicating mucins importance in intestinal barrier repair
