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HUVECs were cultured until the sixth passage in 0.2% pre-coated gelatin (Sigma, cat
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Collectively, they suggest four principal ways through which CS imposes ROS burden on biological systems: (1) ROS, as well as radicals, are intrinsically present in CS, (2) CS constituents can induce ROS generation via chemical reactions with biomolecules, (3) CS can stimulate endogenous ROS sources in cells to produce ROS, and (4) CS can interfere with antioxidant systems, thereby exacerbating the production or functions of ROS (Figure 1)
We first validated the role of GCLC succinylation in ferroptosis by inactivating GCLC with shRNA or BSO in ACHN cells

Indeed, mTORC1 promotes Wnt-driven tumor initiation but limits colon cancer development upon chronic inflammation
