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Therefore, there are occasions when a B12 deficiency can occur without anemia, megaloblastosis, or initial symptoms if there is a high intake of folates, which further complicates early diagnosis and can worsen symptoms, as symptoms (many of which are serious) may appear suddenly at a later stage, having been masked by the deficiency until then

For extraction of DNA adducts, Strata X SPE cartridges (200 mg/6 ml) were conditioned with 12 ml of acetone followed by 12 ml of methanol and equilibrated with 12 ml of distilled water