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Immunofluorescence Assay Cells grown on coverslip were fixed with 4% paraformaldehyde in PBS for 20 min and permeabilized by 0.1% Triton X100 for 15 min at room temperature

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No increase in the risk of amputations or ketoacidosis was reported

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Step 7: Remove air bubbles With the vial still inverted and the needle still inside, check for air bubbles in the syringe

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